Analysis of CSRNP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1486-LGZ)

Name: Analysis of CSRNP3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1486-LGZ
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Introduction

Official Full Name: cysteine and serine rich nuclear protein 3
Also known as: TAIP2; TAIP-2; PPP1R73; FAM130A2
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and sequence-specific DNA binding activity. Predicted to be involved in positive regulation of apoptotic process and positive regulation of transcription by RNA polymerase II. Predicted to be part of chromatin. Predicted to be active in nucleus.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.