Analysis of CSGALNACT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1920-LGZ)

Name: Analysis of CSGALNACT1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1920-LGZ
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Introduction

Official Full Name: chondroitin sulfate N-acetylgalactosaminyltransferase 1
Also known as: ChGn; ChGn-1; SDJLABA; CSGalNAcT-1; beta4GalNAcT
This gene encodes an enzyme that transfers N-acetylglucosamine (GalNAc) to the core tetrasaccharide linker and to elongating chondroitin sulfate chains in proteoglycans. Knockout of the orthologous mouse gene indicates that the protein is necessary for normal cartilage development and aggrecan metabolism. Mutations in this gene are associated with multiple sclerosis progression, and with mild skeletal dysplasia and joint laxity.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.