Analysis of CPLANE2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1302-LGZ)

Name: Analysis of CPLANE2 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1302-LGZ
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Introduction

Official Full Name: ciliogenesis and planar polarity effector complex subunit 2
Also known as: RSG1; C1orf89
Predicted to enable GTP binding activity and GTPase activity. Predicted to be involved in cellular protein localization; cilium assembly; and regulation of vesicle-mediated transport. Predicted to be located in ciliary basal body.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.