Analysis of CORT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1303-LGZ)

Name: Analysis of CORT Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1303-LGZ
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Introduction

Official Full Name: cortistatin
Also known as: SST2; CST-14; CST-17; CST-29
This genetically encoded neuropeptide is structurally similar to somatostatin. It binds to all known somatostatin receptors and shares many pharmacological and functional properties with somatostatin, including inhibition of neuronal activity. However, it also has many properties distinct from somatostatin, such as inducing slow-wave sleep, apparently by antagonizing the excitatory effects of acetylcholine on the cortex, reducing locomotor activity, and activating cation-selective currents that are unresponsive to somatostatin. The preproproteins are further processed into various mature products. There is a readable transcript between this gene and the upstream APITD1 (apoptosis-inducing, taf9-like domain 1) gene.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.