Analysis of Complement C9 (Human) by ELISA (CAT#: STEM-MB-0753-LGZ)

Name: Analysis of Complement C9 (Human) by ELISA
SKU: STEM-MB-0753-LGZ
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Introduction

The complement system is composed of more than 30 kinds of plasma proteins and membrane proteins, widely present in blood, interstitial fluid and cell surface, and is a protein reaction system with precise regulation mechanism. The main physiological function of complement is to promote the phagocytosis of phagocytes and dissolve target cells, so it is an important part of the body's immune defense mechanism.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.