Analysis of CLIC3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2023-LGZ)

Name: Analysis of CLIC3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-2023-LGZ
Rating: 5 (1 reviews)

Introduction

Official Full Name: chloride intracellular channel 3
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes, including stabilization of membrane potential, epithelial trafficking, maintenance of intracellular pH, and regulation of cell volume. Intracellular chloride channel 3, a member of the p64 family, localizes primarily to the nucleus and stimulates chloride channel activity. Furthermore, based on its association with ERK7, a member of the MAP kinase family, this protein may be involved in cell growth control.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.