Analysis of CD247 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0825-LGZ)

Name: Analysis of CD247 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0825-LGZ
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Introduction

Official Full Name: CD247 molecule
Also known as: T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3ZETA; CD3-ZETA
The protein encoded by this gene is the T-cell receptor zeta, which together with the T-cell receptor alpha/beta and gamma/delta heterodimers and CD3-gamma, -delta and -epsilon form the T-cell receptor-CD3 complex. Zeta chains play an important role in the coupling of antigen recognition to multiple signal transduction pathways in cells. Low expression of antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been identified.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.