Analysis of CASZ1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1290-LGZ)

Name: Analysis of CASZ1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1290-LGZ
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Introduction

Official Full Name: castor zinc finger 1
Also known as: CST; SRG; CAS11; ZNF693; dJ734G22.1
The protein encoded by this gene is a zinc finger transcription factor. The encoded protein may function as a tumor suppressor, and single nucleotide polymorphisms in this gene are associated with blood pressure variation. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.