Analysis of CA1 (Human) by ELISA (CAT#: STEM-MB-0707-LGZ)

Name: Analysis of CA1 (Human) by ELISA
SKU: STEM-MB-0707-LGZ
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Introduction

Carbonic anhydrases (CA) are a large class of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They exhibit wide diversity in tissue distribution and subcellular localization. The CA1 gene is closely related to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein found at the highest level in red blood cells.

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Principle Applications Procedure Materials

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.