Analysis of Bcl-2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0053-LGZ)

Introduction

Bcl-2 gene (namely B-cell lymphoma/leukemia-2 gene) is an oncogene, which can obviously inhibit cell apoptosis, and some studies in recent years have begun to reveal the mechanism of this effect. Bcl-2 is the main target molecule in the study of apoptosis molecular mechanism. With the deepening of the research on Bcl-2 and apoptosis itself, the mechanism of action of Bcl-2 and the molecular mechanism of apoptosis are finally elucidated, thereby improving the understanding, diagnosis and treatment of diseases related to apoptosis.

STEMart technology detail



Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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