Analysis of ALDH9A1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0794-LGZ)

Name: Analysis of ALDH9A1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0794-LGZ
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Introduction

Official Full Name: aldehyde dehydrogenase 9 family member A1
Also known as: E3; ALDH4; ALDH7; ALDH9; TMABADH; TMABA-DH; TMABALDH
This protein belongs to the aldehyde dehydrogenase family of proteins. It has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. The enzyme catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA). This isozyme is a tetramer of identical 54-kD subunits.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.