Analysis of AGXT Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1556-LGZ)

Name: Analysis of AGXT Gene (Mutation) by RT-qPCR
SKU: STEM-MT-1556-LGZ
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Introduction

Official Full Name: alanine--glyoxylate aminotransferase
Also known as: AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1; Ser-PyrAT
This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.