Analysis of Adjuvants induced antigen alteration by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0797-WXH)

Name: Analysis of Adjuvants induced antigen alteration by Differential Scanning Fluorimetry(DSF)
SKU: STEM-MB-0797-WXH
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Introduction

An adjuvant is a substance that enhances the immune system’s response to the presence of an antigen. They are commonly used to improve the effectiveness of a vaccine. Generally, they are injected alongside an antigen to help the immune system generate antibodies that fight the antigen. Adjuvants are important to enhance the antigens immunogenicity, but may also alter the structures of antigens.

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Principle Applications Procedure Materials

Principle

Differential Scanning Fluorimetry measures protein thermal unfolding by monitoring changes in fluorescence emission of a sample upon heating. This allows the determination of protein thermostability and complex formation even with weakly binding ligands by thermal shift assay. Differential Scanning Fluorimetry is therefore ideally suited for screening of optimum buffer conditions like pH, buffer composition and ionic strength. The technique is applicable to any biological sample, from soluble proteins to integral membrane proteins.

Applications

To identify low-molecular-weight ligands that bind and stabilize purified proteins.
To measure the denaturation and unfolding of proteins.

Procedure

1. Preparation of compound solutions
2. Preparation of buffer/additive screen plates
3. Preparation of compound storage plates
4. Equipment preparation
5. Sample preparation
51. Performing the scan