Analysis of ACBD3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0747-LGZ)

Name: Analysis of ACBD3 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0747-LGZ
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Introduction

Official Full Name: acyl-CoA binding domain containing 3
Also known as: PAP7; GCP60; GOCAP1; GOLPH1
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.