Analysis of A2M (Human) by ELISA (CAT#: STEM-MB-0643-LGZ)

Introduction

Alpha-2-macroglobulin (A2M) is a proteinase inhibitor. A2M acts as a broad spectrum proteinase binding protein. It is a large plasma glycoprotein and has long been recognized as an irreversible inhibitor of many proteases. Recently, many growth factors, cytokines, and hormones have been reported to bind to α2M through a variety of mechanisms. A2M is also produced in the brain, where it binds to a variety of extracellular ligands and is internalized by neurons and astrocytes. In the brains of patients with Alzheimer's disease (AD), A2M is localized to diffuse amyloid plaques. A2M also binds to soluble beta-amyloid protein, where it mediates degradation. Clinical cirrhosis, nephrotic syndrome, diabetes, severe burn cases increased.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

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