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Analysis Interactions of Endosidin2 and Arabidopsis thaliana EXO70A1 (AtEXO70A1) by Microscale Thermophoresis (MST) (CAT#: STEM-MB-2437-LGZ)
Introduction
Microscale thermophoresis (MST) is a biophysical analysis method for quantifying interactions between molecules, such as proteins and small molecules. In recent years, the MST method has been used to detect protein-protein and protein-drug interactions. The method detects molecular interactions by quantifying the thermophoretic motion of fluorescent molecules in response to a temperature gradient.
Principle
One of the interacting molecules (mostly proteins) is labeled with fluorescent dye or combined with GFP label. The labeled protein and ligand molecules are placed in the capillary according to a specific concentration gradient. Infrared laser heating generates a microscopic temperature gradient field to undergo thermophoresis, with which the hydration layer, molecular size, electric charge and other molecular properties will change. Then the fluorescence distribution in the reaction system changes. In addition to accurately detecting interactions between biomolecules, MST can also obtain other parameters related to interactions between biomolecules by calculating dissociation constants (Ks), and achieve accurate qualitative analysis.
Applications
For characterizing thermodynamic parameters of biomolecular interactions.
Procedure
1. Sample processing.
2. MST detection.
3. Data analysis.
2. MST detection.
3. Data analysis.
Materials
• Sample Type: protein 10uM/ 100ul or 50 micrograms, small molecules 100uM/200ul, protein peptides need to be sent at low temperature
Notes
In order to ensure the reliability of the test, some samples should be provided as much as possible.
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