High-quality products
Reliable performance
- Home
- Technical Services
- Intermolecular Interaction Detection
- Bio-Layer Interferometry (BLI) Technology
Analysis Biomolecular Interactions of Aptamers (oligdT and TATA dsDNA) with BPA-HRP by BLI (CAT#: STEM-MB-0113-CJ)
Introduction
Many polycarbonate plastics contain bisphenol A (BPA), which is one of the most widely produced chemicals in the world. It is used in hard plastics, food cans, drink cans, receipts, and dental sealants. BPA is a small carcinogenic molecule (MW = 228 Da) linked to breast and prostate cancer. BPA is regarded as an endocrine disruptor because it can mimic the action of hormone estrogen and disturb the estrogen–estrogen receptor binding process (hormonal pathways). And L-DNAzyme- and L-DNA-templated nanoparticles or tetrahedron nanostructures were used for metal ion sensing and molecular imaging and as anticancer drug delivery vehicles.
Principle
Bio-Layer Interferometry (BLI) is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. BLI experiments are used to determine the kinetics and affinity of molecular interactions. In a BLI experiment, one molecule is immobilized to a Dip and Read Biosensor and binding to a second molecule is measured. A change in the number of molecules bound to the end of the biosensor tip causes a shift in the interference pattern that is measured in real-time.
Applications
Oncology & Cancer; Immunology/Inflammation; Pharmacology
Procedure
1. Detect Buffers and prepare samples. BLI experiments are set up with one molecule immobilised on the surface of the biosensor (load sample) and a second molecule in solution (the analytical sample).
2. Fix the load sample on the biocompatible biosensor while the analytical sample is in solution.
3. The biosensor tip is immersed in the solution so that the target molecule begins to bind to the analysis sample.
4. Set up and run the BLI experiment. Molecules bound to or dissociated from the biosensor can generate response curves on the BLI system; unbound molecules, changes in the refractive index of the surrounding medium or changes in flow rate do not affect the interferogram pattern.
5. Collect and analyse data on the BLI's system.
2. Fix the load sample on the biocompatible biosensor while the analytical sample is in solution.
3. The biosensor tip is immersed in the solution so that the target molecule begins to bind to the analysis sample.
4. Set up and run the BLI experiment. Molecules bound to or dissociated from the biosensor can generate response curves on the BLI system; unbound molecules, changes in the refractive index of the surrounding medium or changes in flow rate do not affect the interferogram pattern.
5. Collect and analyse data on the BLI's system.
Materials
• Equipment: Fortebio Bio-Layer Interferometry (BLI)
• Sample Type: DNA, RNA, Protein, Antibodies, Peptides, Small Molecules
• Optionals: BPA, DMSO, Anti-BPA Antibody, Triple Deionized Distilled Water
• Sample Type: DNA, RNA, Protein, Antibodies, Peptides, Small Molecules
• Optionals: BPA, DMSO, Anti-BPA Antibody, Triple Deionized Distilled Water
Other recommended products
-
Analysis Biomolecular Interactions of Antigen with Cell-Free Produced Samples by BLI (CAT#: STEM-MB-0141-CJ)
-
-
Analysis Biomolecular Interactions of Antibody with Kallikrein-related Peptidase 7 (KLK7) by BLI (CAT#: STEM-MB-0100-CJ)
-
Analysis Kinetics of mAb Panel's Avidity to Peptide 22 by BLI (CAT#: STEM-MB-0281-CJ)
-
Analysis Biomolecular Interactions of PTH-Fc and PTH1R by BLI (CAT#: STEM-MB-0152-CJ)
-
-
Analysis Biomolecular Interactions of Ku70 Domain β-barrel with HBoV1 NP1 by BLI (CAT#: STEM-MB-0197-CJ)
-
Analysis Biomolecular Interactions of P RNA and P protein with Individual Compounds by BLI (CAT#: STEM-MB-0138-CJ)
Wide range of applications
For various laboratory needs
Expert support
Professional service team
Fast delivery
Global shipping