Analysis Affinity of HGF and AMG 102 by Kinetic. (CAT#: STEM-MB-0005-CJ)

Introduction

Hepatocyte growth factor [HGF; also known as scatter factor (SF) ] is the only known ligand for c-Met, a receptor tyrosine kinase expressed in epithelial tissues that plays an essential role in the growth and maintenance of cells. Signaling through HGF/SF:c-Met mediates a number of normal cellular functions, including proliferation, survival, motility, migration, angiogenesis, and morphogenesis. Abnormal signaling via the HGF/SF:c-Met pathway has been associated with many types of cancers, with evidence for both paracrine and autocrine activation of c-Met by HGF; expression of these molecules in tumors and elevated concentrations of circulating HGF have been associated with an increased risk of tumor metastasis and poor prognosis.<br /><br />AMG 102 is a fully human monoclonal antibody that selectively targets and neutralizes hepatocyte growth factor/scatter factor (HGF/SF). AMG 102 also inhibited cynomolgus monkey HGF-induced migration of human MDA-MB-435 cells but not rat HGF-induced migration of mouse 4T1 cells.