Analysis Affinity of AMG 139 to IL-23 by Kinetic (CAT#: STEM-MB-0003-CJ)

Introduction

IL-23 is a member of the IL-12 family of heterodimeric cytokines and is composed of the IL-23 specific p19 subunit and the common subunit p40, which it shares with IL-12. Similarly, the heterodimeric cell surface transmembrane IL-12 and IL-23 receptor complexes share a common subunit, IL-12 receptor β1 subunit, that associates with IL-12 receptor β2 subunit or the IL-23 receptor for IL-12 and IL-23 signalling, respectively, which allows for control of distinct biological pathways. IL-23 are produced by antigen presenting cells such as dendritic cells and macrophages in response to inflammation or infection. IL-23 acts on immune cells, including T-helper 17, γδ T-cells, natural killer (NK) cells, dendritic cells, macrophages and innate lymphoid cells to induce production of cytokines such as IL-17, IL-6, TNF-α and GM-CSF. Increased expression of IL-23 is found in the target tissue of inflammatory/autoimmune diseases including Crohn’s disease, ulcerative colitis, psoriasis, rheumatoid arthritis and<br />multiple sclerosis. AMG 139 is a human anti-IL-23 antibody currently in a phase II trial for treating Crohn's disease.