AMMS® NK Cell Culture Kit, Seafrom (CAT#: STEM-CT-0162-ZJF)

The AMMS® NK Cell Culture Kit manufactured by Seafrom is suitable for the cultivation of natural killer (NK) cells, providing a comprehensive kit for the optimal growth and activation of NK cells.
This product is suitable for the in vitro expansion of highly purified NK cells obtained from autologous peripheral blood or cord blood PBMCs after in vitro activation. It is intended for research purposes only and not for clinical use.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: CD223, FDC
Protein Sequence: The DNA sequence encodes human LAG3 (NCBI Reference Sequence: NP_001762.2), expressed with an Fc tag at the C-terminus.
Molecular Weight: Recombinant human LAG-3 protein contains 689 amino acids, with a predicted theoretical molecular weight of 75.6 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: Immobilized anti-LAG-3 (Fc tag) antibody at 5 μg/mL (100 μL/well) can bind to human FGL-1 (His tag) in a linear range of 50-200 ng/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.
NK Cell Culture Kit:

Kit Components:
NK Reagent A: 200 μL, 1 vial, stored at -20℃
NK Reagent B: 500 μL, 1 vial, stored at -20℃
NK Reagent C: 500 μL, 1 vial, stored at -20℃
NK Reagent D: 500 μL, 1 vial, stored at -20℃
NK Reagent E: 500 μL, 1 vial, stored at -20℃
NK Serum-Free Culture Medium:
NK Serum-Free Culture Medium: 1000 mL, 2 bottles, stored at 2-8℃.

Serum-Free Culture with Pure Factor Method
High Activation and Expansion Efficiency
Self-Produced Reagent Kit Components with High Batch Stability
ISO13485 Medical Device Quality System Certification
Gmp Pharmaceutical-Grade Production Environment

Recombinant Human LAG-3 Protein (His), Seafrom
Recombinant Human LAG-3 (Fc) Protein, Seafrom
AMMS® HEK293 Feed Medium, Seafrom
AMMS® NK Cell Culture Kit 2.0, Seafrom