Almond-Derived Component Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0042-ZJF)

Highlights

Ready-to-use kit requiring only user-supplied DNA template.
High specificity: Primers are designed based on highly conserved regions of almond-derived DNA and do not amplify non-almond components.
Optimized primers and amplification system offer sensitivity 100 times higher than conventional PCR.

Cat Number: STEM-SLSR-0042-ZJF

Application: For qualitative and quantitative analysis of Almond-Derived Component

Model: 25207




Description

This kit offers a sensitive and specific method for detecting Almond-Derived Component using SYBR Green-based real-time PCR. Designed with primers targeting conserved regions, the assay produces fluorescence signals correlating with amplification levels. The real-time monitoring capability ensures accurate qualitative and quantitative results.

Specification

Kit Components:
Almond-Derived Component Amplification Mix: 50 µL × 1 tube
Almond-Derived Component Reaction Mix: 950 µL × 1 tube
Almond-Derived Component Positive Control: 50 µL × 1 tube
Almond-Derived Component Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Features

Ready-to-use kit requiring only user-supplied DNA template.
High specificity: Primers are designed based on highly conserved regions of almond-derived DNA and do not amplify non-almond components.
Optimized primers and amplification system offer sensitivity 100 times higher than conventional PCR.
Includes non-infectious PCR positive control to help identify false-negative samples.
Closed one-tube fluorescence PCR workflow minimizes the risk of environmental contamination.
For research use only; kit provides sufficient reagents for 50 PCR reactions with a 2 μL reaction volume.

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