P1231 HiPure Plasmid EF Midi Kit (CAT#: STEM-GT-0119-ZJF)

The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Main Functions: Isolation up to 500µg endotoxin-free plasmid DNA from 25-50ml bacterial culture
Applications: Cell transfection, animal injection
Purification method: Midi spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: High copy plasmid vector
Sample amount: 25-50ml LB
Yield: 10-500μg
Elution volume: ≥0.1ml
Time per run: ≤60 minutes
Liquid carrying volume per column: 4ml
Binding yield of column: 250μg

High purity: purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast: silica gel column purification is much faster than ion exchange
High concentration: easily obtain concentrations >1000ng/μl
Small amount large extraction: using a small amount of column to achieve a large extraction yield (500μg)
Low endotoxin content: the obtained plasmid can be directly used in cell transfection and animal injection

STEM-GT-0117-ZJF
STEM-GT-0118-ZJF
STEM-GT-0120-ZJF
STEM-GT-0121-ZJF