P1116 HiPure Plasmid EF Mega Kit (CAT#: STEM-GT-0115-ZJF)

The HiPure Plasmid DNA Mega Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 10 mg high copy number plasmid DNA can be purified from 500 mL overnight culture.

Main Functions: Isolation up to 10mg endotoxin-free plasmid DNA from 500ml bacterial culture
Applications: Cell transfection, animal injection
Purification method: Mega spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: High copy plasmid vector
Sample amount: 500ml LB
Yield: 1-10mg
Elution volume: ≥2ml
Time per run: ≤70 minutes
Liquid carrying volume per column: 50ml
Binding yield of column: 10mg

High purity: purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast: silica gel column purification is much faster than ion exchange
High yield: up to 10mg plasmid can be binded in one column
Low endotoxin – the obtainedplasmid can be directly used for cell transfection and animal injection, etc.

STEM-GT-0113-ZJF
STEM-GT-0114-ZJF
STEM-GT-0116-ZJF
STEM-GT-0117-ZJF