R4163 HiPure Liquid RNA (miRNA) Kit (CAT#: STEM-GT-0082-ZJF)

Highlights

High quality: high purity total RNA can be directly used in various sensitive downstream applications
Fast – isolation of several samples can be completed in 40 minutes by using column purification method
Safety: no phenol chloroform extraction required

Cat Number: STEM-GT-0082-ZJF

Application: Isolation total RNA(include miRNA) from 0.25ml whole blood(fresh or frozen blood)/plasma/serum/body fluids using column and MagZol LS reagent

Model: R4163

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Description

The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.

Specification

Main Functions: Isolation total RNA from 0.25ml body fluids using column and MagZol LS reagent
Applications: qPCR / RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection
Purification method: Mini spin column
Purification technology: Silica technology, acid phenol / guanidine extraction technology (MagZol pretreatment technology)
Process method: Manual (centrifugation or vacuum)
Sample type: Blood, serum, plasma
Sample amount: 250μl
Elution volume: ≥20μl
Liquid carrying volume per column: 800µl
Binding yield of column: 100μg

Features

High quality: high purity total RNA can be directly used in various sensitive downstream applications
Fast – isolation of several samples can be completed in 40 minutes by using column purification method
Safety: no phenol chloroform extraction required
Sensitive: direct lysis of blood, plasma and other samples without separation of leukocytes

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