D3181 HiPure Circulating Nucleic acid Mini Kit (CAT#: STEM-GT-0014-ZJF)

Highlights

High yield: most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration: low elution volume, ensuring high nucleic acid concentration
High purity: low alcohol binding method, completely removing inhibitor and protein pollution

Cat Number: STEM-GT-0014-ZJF

Application: Isolation Circulating/Cell free/CfDNA from 1ml plasma, serum, body fluids

Model: D3181

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Description

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Mini Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be fresh or frozen (provided that they have not been frozen and thawed more than once).

Specification

Main Functions: Isolation circulating DNA from 1ml plasma, serum, body fluids
Applications: qPCR, liquid/solid chip analysis, hybridization, SNP detection
Purification method: Mini spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Serum, plasma, other cell-free fluid samples
Sample amount: 1ml
Elution volume: ≥30μl
Time per run: ≤50 minutes
Liquid carrying volume per column: 800µl
Binding yield of column: 100µg

Features

High yield: most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration: low elution volume, ensuring high nucleic acid concentration
High purity: low alcohol binding method, completely removing inhibitor and protein pollution
High recovery: DNA can be recovered at the level of PG by silica gel column purification

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