R4182 HiPure Microbial RNA Kit (CAT#: STEM-GT-0089-ZJF)

Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

Main Functions: Isolation total RNA from bacteria, yeast cells
Applications: RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method: Mini spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Bacteria, yeast cells
Sample amount: Bacteria <10^9; Yeast cells <2 x 10^7
Elution volume: ≥30μl
Time per run: ≤40 minutes
Liquid carrying volume per column: 800µl
Binding yield of column: 100μg

Fast: several samples can be extracted in 30 minutes
High purity: the purified RNA can be directly used in various downstream applications
High recovery: RNA can be recovered at the level of PG
Good repeatability: silica gel column purification technology can obtain ideal results every time
Broad spectrum: it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
Sufficient components: the kit contains carried wall breaking enzymes and glass beads

STEM-GT-0087-ZJF
STEM-GT-0088-ZJF
STEM-GT-0090-ZJF
STEM-GT-0091-ZJF