R4161 HiPure Blood RNA Mini Kit (CAT#: STEM-GT-0081-ZJF)

Highlights

High quality: high purity total RNA can be directly used in various sensitive downstream applications
Fast: isolation of several samples can be completed in 20 minutes by using column purification method
Sensitive: RNA can be purified at the level of PG

Cat Number: STEM-GT-0081-ZJF

Application: Isolation total RNA from 1-1.5ml whole blood

Model: R4161

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Description

The Kit provides fast purification of high-quality RNA from whole blood, cells and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol / chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection and in vitro translation.

Specification

Main Functions: Isolation total RNA from 1-1.5ml whole blood
Applications: qPCR / RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection
Purification method: Mini spin column
Purification technology: Silica technology, acid phenol / guanidine extraction technology (MagZol pretreatment technology)
Process method: Manual (centrifugation or vacuum)
Sample type: Fresh or frozen blood, mammalian blood
Sample amount: ≤1.5 ml whole blood
Yield: 2-100μg
Elution volume: ≥20μl
Liquid carrying volume per column: 800µl
Binding yield of column: 100μg

Features

High quality: high purity total RNA can be directly used in various sensitive downstream applications
Fast: isolation of several samples can be completed in 20 minutes by using column purification method
Sensitive: RNA can be purified at the level of PG

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