R4111 HiPure Total RNA Plus Kit (CAT#: STEM-GT-0065-ZJF)

This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Main Functions: Isolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 x 10^6 cell using two columns (gDNA removed column)
Applications: RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification method: Mini spin column
Purification technology: Silica technology, DNA filtration technology
Process method: Manual (centrifugation or vacuum)
Sample type: Animal soft tissue, cultured cells, lymphocytes, simple plant tissue
Sample amount: Cells ≤1 x 10^7, Animal tissue sample 1-20 mg, Plant tissue 50-150 mg
Yield: 2-100μg
Elution volume: ≥50μl
Time per run: ≤25 minutes（1-24 samples）
Liquid carrying volume per column: 800µl
Binding yield of column: 100µg

Efficient removal of DNA: unique genomic DNA removal column without DNase treatment
High quality: high purity total RNA can be directly used in various sensitive downstream applications
Fast: several samples can be extracted in 25 minutes by column method
Safe: no phenol chloroform extraction required
Sensitive: RNA can be recovered at the level of PG

STEM-GT-0063-ZJF
STEM-GT-0064-ZJF
STEM-GT-0066-ZJF
STEM-GT-0067-ZJF