R4130 HiPure Universal RNA Kit (CAT#: STEM-GT-0066-ZJF)

The Kit integrates phenol/guanidine-based lysis and silica membrane purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg animal, plant, fungal samples,bacteria, cells and other samples.

Main Functions: Isolation total RNA (include miRNA) from 100mg lipid tissue, tissue, cell, plant, body fluids using columns and MagZol reagent
Applications: RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification method: Mini spin column
Purification technology: Silica technology, acid phenol / guanidine extraction technology (MagZol pretreatment technology)
Process method: Manual (centrifugation or vacuum)
Sample type: Animal tissue, muscle fiber containing tissue, adipose tissue, cultured cells, lymphocytes, simple plants and other biological samples
Sample amount: Animal tissue sample 1-60mg (spleen≤10mg), Cultured cells 5 x 10^6, Plant leaves 50-100mg
Yield: 2-200μg
Elution volume: ≥50μl
Time per run: ≤30 minutes（1-24 samples）
Liquid carrying volume per column: 800µl
Binding yield of column: 100µg

Efficient DNA removal: one step RNA extraction can effectively remove genomic DNA
High quality: one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast: several samples can be extracted in 30 minutes
High applicability: samples including animals, plants, bacteria, cells, etc.
High output: enable to process large samples and the yield can be up to 200μg

STEM-GT-0064-ZJF
STEM-GT-0065-ZJF
STEM-GT-0067-ZJF
STEM-GT-0068-ZJF