R4316 HiPure Circulating DNA/RNA Kit (CAT#: STEM-GT-0084-ZJF)

Highlights

High yield: most optimized process to obtain maximum free RNA and small RNA
High concentration: low elution volume (>20μl) to ensure nucleic acid concentration
High purity: low alcohol combination, completely remove inhibitor and protein pollution

Cat Number: STEM-GT-0084-ZJF

Application: Isolation both Circulating DNA/RNA (including miRNA) from 1-5 ml serum and plasma

Model: R4316

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Description

This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection.
HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.

Specification

Main Functions: Isolation both Circulating DNA/RNA (include miRNA) from 1-5 ml serum and plasma
Applications: qPCR / RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection
Purification method: Midi spin column
Purification technology: Silica technology, DNA filtration technology
Process method: Manual (centrifugation or vacuum)
Sample type: serum, plasma, and other cell-free liquid samples
Sample amount: 1-5 ml
Elution volume: ≥20μl
Time per run: ≤100 minutes
Liquid carrying volume per column: 4 ml
Binding yield of column: 1 mg

Features

High yield: most optimized process to obtain maximum free RNA and small RNA
High concentration: low elution volume (>20μl) to ensure nucleic acid concentration
High purity: low alcohol combination, completely remove inhibitor and protein pollution
High recovery: silica gel column technology can recover nucleic acid molecules at the level of PG
Large volume: 1-2ml serum and plasma samples can be processed at one time

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