This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.
Specification
Main Functions: Isolation total RNA from FFPE tissue Applications: RT-PCR, cDNA synthesis, second generation sequencing Products: RNA, miRNA Purification method: Polydisperse magnetic beads Purification technology: Magnetic beads technology Process method: Manual or automatic Adaptive instrument: Nucleic acid extractor, pipetting workstation Sample type: FFPE slice, FFPE embedded tissue Sample amount: ≤6 slices
Features
Fast: several samples can be extracted in 120 minutes by column method Safe: no phenol chloroform extraction required