R4181 HiPure Bacterial RNA Kit (CAT#: STEM-GT-0088-ZJF)

HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.

Main Functions: Isolation total RNA from bacteria culture
Applications: RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method: Mini spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Bacteria culture
Sample amount: Bacteria <10^9
Elution volume: ≥30μl
Time per run: ≤40 minutes
Liquid carrying volume per column: 800µl
Binding yield of column: 100μg

Fast: several samples can be extracted in 30 minutes
High purity: the purified RNA can be directly used in various downstream applications
High recovery: RNA can be recovered at the level of PG
Good repeatability: silica gel column purification technology can obtain ideal results every time
Broad spectrum: it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
Sufficient components: the kit contains carried lysozyme and glass beads

STEM-GT-0086-ZJF
STEM-GT-0087-ZJF
STEM-GT-0089-ZJF
STEM-GT-0090-ZJF