P1811 MagPure Plasmid Mini Kit (CAT#: STEM-GT-0120-ZJF)

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Main Functions: Isolation up to 20µg plasmid DNA from 1-3ml bacterial culture
Applications: Enzyme digestion, sequencing, PCR, labeling
Purification technology: Magnetic beads technology
Process method: Manual or automatic
Sample type: Conventional plasmid, plasmid≤30KB
Sample amount: 1-3ml
Elution volume: ≥50μl

Suitable for extracting plasmids from 1-5ml or <3ml YT medium.
The same amount of buffer 1, 2, and 3 avoids errors caused by adjusting the pipette, making it convenient to use in conjunction with automated workstations.
Containing buffer 1 for washing, reducing the problem of false high production.
The purified plasmid can be directly used for sequencing, enzyme digestion, PCR, and other applications.

STEM-GT-0118-ZJF
STEM-GT-0119-ZJF
STEM-GT-0121-ZJF
STEM-GT-0122-ZJF