P1014 HiPure Fastfilter Plasmid Maxi Kit (CAT#: STEM-GT-0114-ZJF)

The HiPure FastFilter Plasmid DNA Maxi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes a special filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 1000 µg high copy number plasmid DNA or 50-500 µg low copy number plasmid DNA can be purified from 200mL overnight culture.

Main Functions: Isolation up to 1mg plasmid DNA from 200ml bacterial culture
Applications: Enzyme digestion, sequencing, PCR, labeling
Purification method: Maxi spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Conventional plasmid, plasmid less than 30KB
Sample amount: 100-200ml
Yield: 0.4-1mg
Elution volume: ≥500μl
Time per run: ≤60 minutes
Liquid carrying volume per column: 20ml
Binding yield of column: 1mg

High purity: purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast: it takes only 15-60 minutes to complete the isolation
Economy: high cost performance

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