P1814 MagPure Plasmid EF Mini Kit (CAT#: STEM-GT-0121-ZJF)

Highlights

High purity: purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast: it takes only 80 minutes to complete the isolation
High yield: up to 15μg plasmid can be binded in one column

Cat Number: STEM-GT-0121-ZJF

Application: Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture

Model: P1814

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Description

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Specification

Main Functions: Isolation up to 15μg endotoxin-free plasmid DNA from 1-5ml bacterial culture
Applications: Enzyme digestion, sequencing, PCR, labeling
Purification technology: Magnetic beads technology
Process method: Manual or automatic
Sample type: Conventional plasmid, plasmid≤30KB
Sample amount: 1-5ml
Elution volume: ≥50μl
Time per run: ≤80 minutes

Features

High purity: purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast: it takes only 80 minutes to complete the isolation
High yield: up to 15μg plasmid can be binded in one column
Economic: excellent cost effectiveness performance

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