P1013 HiPure Fastfilter Plasmid Midi Kit (CAT#: STEM-GT-0113-ZJF)

The HiPure Fastfilter Plasmid DNA Midi Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. This system also includes aspecial filter cartridge which replaces the centrifugation step following alkaline lysis. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 250 µg high copy number plasmid DNA or 10-75 µg low copy number plasmid DNA can be purified from 15-50 mL overnight culture.

Main Functions: Isolation up to 250μg plasmid DNA from 30-50ml bacterial culture
Applications: Enzyme digestion, sequencing, PCR, labeling
Purification method: Midi spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Conventional plasmid, plasmid less than 30KB
Sample amount: 30-50ml
Yield: 50-250µg
Elution volume: ≥300μl
Time per run: ≤60 minutes
Liquid carrying volume per column: 4ml
Binding yield of column: 250µg

High purity: purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast: it takes only 15-60 minutes to complete the isolation
High yield: up to 70µg plasmid can be binded in one column

STEM-GT-0111-ZJF
STEM-GT-0112-ZJF
STEM-GT-0114-ZJF
STEM-GT-0115-ZJF