D3180 HiPure Circulating Nucleic Acid Micro Kit (CAT#: STEM-GT-0013-ZJF)

Free-circulating nucleic acids, such as tumor-specific extracellular nucleic acid fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp(Nucleic Acid). The HiPure Circulating Nucleic acid Micro Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be either fresh or frozen (provided that they have not been frozen and thawed more than once). Free-circulating cell-free DNA, RNA or viral nucleic acids are eluted in Nuclease Free Water, ready for use in amplification reactions or storage at -30 to -15°C. Purified nucleic acids are free of proteins, nucleases, and other impurities.

Main Functions: Isolation circulating DNA from 0.6ml plasma, serum, body fluids
Applications: qPCR, liquid/solid chip analysis, hybridization, SNP detection
Purification method: Mini spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Serum, plasma, other cell-free fluid samples
Sample amount: 0.6ml
Elution volume: ≥30μl
Time per run: ≤40 minutes
Liquid carrying volume per column: 800μl
Binding yield of column: 100μg

High yield: most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration: low elution volume, ensuring high nucleic acid concentration
High purity: low alcohol binding method, completely removing inhibitor and protein pollution
High recovery: DNA can be recoveredat the level of PG by silica gel column purification

STEM-GT-0011-ZJF
STEM-GT-0012-ZJF
STEM-GT-0014-ZJF
STEM-GT-0015-ZJF