R4151 HiPure Plant RNA Mini Kit (CAT#: STEM-GT-0077-ZJF)

Highlights

Efficient removal of DNA: unique genomic DNA removal column without DNase treatment
High quality: high purity total RNA can be directly used in various sensitive downstream applications
Fast: several samples can be extracted in 25 minutes by column method

Cat Number: STEM-GT-0077-ZJF

Application: Isolation total RNA from <150 mg simple plant sample without chloroform

Model: R4151

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Description

The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Specification

Main Functions: Isolation total RNA from <150 mg simple plant sample without chloroform
Applications: RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method: Mini spin column
Purification technology: Silica technology, DNA filtration technology
Process method: Manual (centrifugation or vacuum)
Sample type: Economic crops
Sample amount: ≤150 mg
Elution volume: ≥30μl
Time per run: ≤25 minutes
Liquid carrying volume per column: 800µl
Binding yield of column: 100µg

Features

Efficient removal of DNA: unique genomic DNA removal column without DNase treatment
High quality: high purity total RNA can be directly used in various sensitive downstream applications
Fast: several samples can be extracted in 25 minutes by column method
Safe: no phenol chloroform extraction required
Sensitive: RNA can be recovered at the level of PG
Broad spectrum: various types of plant samples can be processed by diversity of operating procedures

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