D3115 HiPure Tissue&Blood DNA Maxi Kit (CAT#: STEM-GT-0004-ZJF)

Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by STEMart Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, tissue and culture cells.

Main Functions: Isolation total DNA from 10ml blood and 1g tissue using Maxi column
Applications: PCR, southern bolt, virus detection
Purification method: Maxi spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Tissue, cell, blood, saliva, swab, blood spot, semen, other clinical samples
Sample amount: 3-10ml
Elution volume: ≥700μl
Time per run: ≤90 minutes
Liquid carrying volume per column: 4ml
Binding yield of column: 5mg

High quality DNA: meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast: without separation of leukocytes, organic extraction or ethanol precipitation
Simple: all nucleic acids can be obtained by direct digestion
Pertinence: specially designed for isolating DNA from 3-10ml blood and related body fluids
Wide applicability: handle a variety of liquid samples
Economy: excellent cost effectiveness performance

STEM-GT-0002-ZJF
STEM-GT-0003-ZJF
STEM-GT-0005-ZJF
STEM-GT-0006-ZJF