R4801 MagZol Reagent (CAT#: STEM-GT-0073-ZJF)

MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) and cells (<5 X106), and large quantities of tissue (up to 1g) and cells (<108), of human, animal, plant, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, poly(A) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.

Main Functions: RNA isolation solvent (substitution for Trizol/Qiazol reagent)
Applications: RT-PCR, Northern hybridization, poly (a) enrichment
Purification technology: Acid phenol guanidine isothiocyanate
Process method: Manual (centrifugation)
Sample type: Animal tissue, plant tissue, adherent cells, suspended cells, bacteria, trace fungi
Sample amount: Flexible
Elution volume: Variation with sample size
Time per run: Variation with sample size

Extensive: suitable for various kinds of biological samples, including animals, plants, cultured cells, bacteria, etc.
High yield: efficient cleavage releases more RNA
High purity: purified RNA is suitable for various downstream applications
Flexible: sample amount can be adjusted according to the demand
High cost performance compared with similar products

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