R4310 HiPure Universal miRNA Kit (CAT#: STEM-GT-0093-ZJF)

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples. Alternatively, a miRNA-enriched fraction and a total RNA (>200nt) fraction can be purified separately. This Kit combines phenol/guanidine-based lysis of samples and silica membrane–based purification of total RNA. MagZol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.

Main Functions: Isolation miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, using MagZol reagent and column
Applications: RT-PCR, Northern Blot, poly A+ purification, nucleic acid protection and in vitro translation
Purification method: Mini spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Animal tissues, adherent cells, suspension cells, bacteria
Sample amount: Eukaryotic culture cells ≤ 10^7, Animal tissue <100mg, Yeast culture cells <5 x 10^7, Bacteria <10^9
Elution volume: ≥15μl
Time per run: ≤40 minutes
Liquid carrying volume per column: 800µl
Binding yield of column: 100µg

High quality: one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast: several samples can be extracted in 40 minutes
High applicability: samples including animals, plants, bacteria, cells, etc.
High concentration: efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity

STEM-GT-0091-ZJF
STEM-GT-0092-ZJF
STEM-GT-0094-ZJF
STEM-GT-0095-ZJF