D3018 HiPure Universal DNA Kit (CAT#: STEM-GT-0001-ZJF)

Highlights

High quality DNA: meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast: without separation of leukocytes, organic extraction or ethanol precipitation
Simple: all nucleic acids can be obtained by direct digestion

Cat Number: STEM-GT-0001-ZJF

Application: Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots / sperm

Model: D3018

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Description

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Specification

Main Functions: Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications: PCR, qPCR, southern bolt, virus detection
Purification method: Mini spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Tissue, cell, blood, saliva, swab, blood spot, semen, other clinical samples
Sample amount: Solid tissue 1-10mg, Anticoagulant blood 200μl
Elution volume: ≥20μl
Time per run: 30-60 minutes
Liquid carrying volume per column: 800µl
Binding yield of column: 100µg

Features

High quality DNA: meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast: without separation of leukocytes, organic extraction or ethanol precipitation
Simple: all nucleic acids can be obtained by direct digestion
Wide applicability- handle a variety of liquid samples

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