R4165 HiPure HP Plant RNA Mini Kit (CAT#: STEM-GT-0079-ZJF)

HiPure HP Plant RNA Mini Kit combines guanidine isothiocyanate lysing and silica gel membrane purification technology to simplify total RNA extraction. Ultracentrifugation in CsCl purification and LiCl / ethanol precipitation are not required. The kit uses DNase digestion to completely remove DNA. It is suitable for extracting up to 100μg of total RNA (including miRNA) from plant samples less than 200mg. Several samples can be extracted within 40 minutes. The purified RNA can be directly used for RT-PCR, fluorescent quantitative RT-PCR, Northern hybridization, second generation sequencing, etc.

Main Functions: Isolation total RNA (include miRNA) from <200mg difficult-to-extract plant samples (use low toxicity chloroform substitutes)
Applications: RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method: Mini spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Hard-to-extraction plant samples such as fruit and seed, grape leaves, tea
Sample amount: ≤200 mg
Elution volume: ≥30μl
Time per run: 1-24 samples within 30 minutes
Liquid carrying volume per column: 800µl
Binding yield of column: 100µg

Completely remove DNA by using of DNase
High quality: one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast: several samples can be extracted in 40 minutes by column method
Sensitive: RNA can be recovered at the level of PG
Broad spectrum: various types of plant samples can be processed by diversity of operating procedures

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