D3148 HiPure Microbiome DNA Kit (CAT#: STEM-GT-0044-ZJF)

Highlights

High concentration: the host DNA is digested by dnase, without contamination of host DNA
High recovery: DNA can be recovered at the level of PG
Good repeatability: silica technology can obtain ideal results every time

Cat Number: STEM-GT-0044-ZJF

Application: Isolation gDNA from biological sample and remove host DNA

Model: D3148

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Description

This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.

Specification

Main Functions: Isolation gDNA from biological samples and removal of host DNA
Applications: PCR, southern blot, enzyme digestion
Purification method: Mini spin column
Purification technology: Silica technology
Process method: Manual (centrifugation or vacuum)
Sample type: Culture medium, swab, parasitic blood, tissue, sputum
Sample amount: Various biological fluids 0.5-1ml
Elution volume: ≥50μl
Time per run: ≤60 minutes
Liquid carrying volume per column: 800μl
Binding yield of column: 100μg

Features

High concentration: the host DNA is digested by dnase, without contamination of host DNA
High recovery: DNA can be recovered at the level of PG
Good repeatability: silica technology can obtain ideal results every time
Wide applicability: it can be used in blood, tissue, intestinal contents and other samples

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