MicroSEQ™ 500 16S rDNA PCR Kit, GeneAmp 9700, Veriti Thermal Cycler, Thermo Fisher Scientific (CAT#: STEM-AS-0281-LSY)

MicroSEQ™ 500 16S rDNA PCR Kit, GeneAmp 9700, Veriti Thermal Cycler, model 4348228 is manufactured by Thermo Fisher Scientific. MicroSEQ®500 16S rDNA PCR Kit is the PCR component of MicroSEQ®500 16S rDNA bacterial identification system. It provides an easy-to-use method based on DNA sequence to identify most bacteria. The MicroSEQ®500 16S rDNA sequencing kit can be used to sequence the PCR amplification products generated by this kit. Together, the two kits can quickly and easily amplify and sequence 500 base pairs of 16S rRNA genes, which can then be compared with the MicroSEQ®16S rDNA 500 library for identity. The kit does not include a copy of the agreement, nor does it include a quick reference card.
The Applied Biosystems MicroSeq system simplifies each step of microbial identification, combining the advantages of MicroSEQ ID software, simple PrepMan Ultra sample preparation reagents and protocols, MicroSeq bacteria and fungi application kits, and industry-leading thermal cyclers and sequencing systems. To ensure the best performance and system-wide integration, we develop and test all components of the system together. For research use only. Not suitable for diagnostic procedures.

Detection Method: Gel Electrophoresis
Applies to (equipment): GeneAmp 9700, Veriti Thermal Cycler
Library: Customizable, Bacterial Library (> 2000 species)
Product Line: MicroSEQ™
Sample type: DNA (Genomic)
Shipping Condition: Dry Ice
Software: MicroSEQ® ID Analysis Software
Target Gene: 1st 500 bp of 16S rRNA gene
Target Molecule: DNA
Target Organism Class: Bacteria
Technology: SBT (Sequence-Based Typing)
Validated Application: Polymerase Chain Reaction
Workflow Step: PCR Amplification
Number: 4348228
Unit specifications: 1 kit
Contents: Each kit contains enough PCR Master Mix to perform 50 PCR amplifications, 5 negative control assays, and 5 positive control assays. One tube of positive control E. coli DNA, and one of water (for use as a negative control) are also included.
Storage: Store at -15 to -25°C upon reciept. After first use, store at 2-6 °C in a PCR Clean Room.

Sequence-based measurements ensure that you get repeatable results within 24 hours.
Measurements that are not related to growth requirements allow you to use objective criteria to identify bacteria.
Universal domain-level primers eliminate pre-optimization.
MicroSEQ ID analysis software ensures accurate species identification.
Customizable libraries give you the flexibility to create new libraries and search for verified libraries containing the most frequently encountered creatures.
The training required is minimal and the workflow is the same as the MicroSEQ D2 LSU Fungal Kit.

Prepman™ Ultra Sample Preparation Reagent, MicroSEQ® ID, Thermo Fisher Scientific (CAT#: STEM-AS-0279-LSY)
Fast MicroSEQ™ 500 16S rDNA PCR Kit, Veriti Thermal Cycler, Thermo Fisher Scientific (CAT#: STEM-AS-0280-LSY)
MicroSEQ™ Full Gene 16S rDNA PCR Kit, GeneAmp 9700, Veriti Thermal Cycler, Thermo Fisher Scientific (CAT#: STEM-AS-0282-LSY)
MicroSEQ™ D2 rDNA Fungal PCR Kit, Veriti Thermal Cyclerr, 9800 Fast Thermal Cycler, Thermo Fisher Scientific (CAT#: STEM-AS-0283-LSY)
MicroSEQ™ ID PurifiCATion Combo Kit V2.0, With Clean-Up Plates, MicroSEQ™, Thermo Fisher Scientific (CAT#: STEM-AS-0284-LSY)
MicroSEQ™ ID PurifiCATion Combo Kit V2.0, With 8-Strips Kit, Microseq™, Thermo Fisher Scientific (CAT#: STEM-AS-0285-LSY)
MicroSEQ™ 500 16S rDNA Sequencing Kit, MicroSEQ™, Thermo Fisher Scientific (CAT#: STEM-AS-0286-LSY)
MicroSEQ™ Full Gene 16S rDNA Sequencing Kit, MicroSEQ™, Thermo Fisher Scientific (CAT#: STEM-AS-0287-LSY)
MicroSEQ™ D2 rDNA Fungal Sequencing Kit, MicroSEQ™, Thermo Fisher Scientific (CAT#: STEM-AS-0288-LSY)