Total Gene Synthesis (CAT#: STEM-MB-0079-WXH)

Introduction

Total gene synthesis refers to the technique of artificially synthesizing double-stranded DNA molecules in vitro. Gene synthesis is to design and synthesize overlapping single-stranded oligonucleotides according to the gene sequence of a certain protein, and then splicing the full length by overlapping extension PCR method. Gene synthesis does not require a template and is one of the means to obtain genes. Its advantages include:<br />1. The synthesis cycle is short and the accuracy rate is high<br />2. Greater flexibility<br />3. Genes that are difficult to obtain or even do not exist in nature can be designed<br />4. Codon optimization can be carried out so that the gene can be well expressed in various biological expression systems