WeSPR One Auto Biomolecular Interaction Analyzer

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WeSPR One Auto

Automation Meets Precision

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WeSPR One Auto Biomolecular Interaction Analyzer (STEMart Original)

The WeSPR One Auto is an automated, single-needle, dual-channel SPR system designed to deliver robust, reproducible interaction data with minimal operator intervention. Leveraging MetaSPR technology, it ensures reliable kinetic and affinity characterization for both purified and complex samples.

Engineered for automation and efficiency, the system supports continuous operation for up to 48 hours unattended, making it an economical and practical solution for laboratories seeking consistent results without constant supervision. With its intuitive setup, WeSPR One Auto simplifies experimental workflows while maintaining high-quality data output, making it particularly suited for small molecule screening and drug discovery research.

Application Areas

Antibody Development
Drug Discovery
Cell Therapy
Gene Therapy
Vaccine Development
Bioproduct Synthesis
In Vitro Diagnostics

WeSPR One Auto Applications (STEMart Original)

Features

WeSPR One Auto Features (STEMart Original)

Automated Workflow: Fully automated sample injection and detection enable continuous 24–48h unattended operation.

Dual-Channel Design: One detection and one reference channel ensure accurate background subtraction for high-quality data.

Flexible Throughput: Supports 1 × 96-well plate with sealing film to minimize sample evaporation during long runs.

Queue Mode Efficiency: Multiple assays can be scheduled and executed automatically, improving productivity and consistency.

Pre-Set Templates: Streamlined method setup reduces preparation time and simplifies routine workflows.

Reliable Performance: Built-in real-time degassing minimizes bubble interference during detection.

High Compatibility: Low bulk effects and wide sample compatibility, including antigens/antibodies, proteins/peptides, nucleic acids, crude extracts, viruses, liposomes, exosomes, cells, supernatants, serum, and plasma.

Specifications

Parameter	Specification
Channels	2 (1 detection, 1 reference)
Applications	Kinetic/affinity characterization, screening, single-cycle kinetics, small molecule interaction analysis, fragment screening, epitope mapping, immunogenicity, concentration analysis (with or without calibration curve), thermodynamics, comparability studies, and sample recovery with MS coupling
Injection Volume	10–500 μL
Flow Rate	5–200 μL/min
Temperature Range	4–40 °C
Sample Throughput	2 × 96-well plates
Buffer Selection	Automated switching between two buffer solutions
Concentration Range	≥1 pM
Association Rate Constant (ka)	Proteins: 10¹–10⁹ M⁻¹s⁻¹; Small molecules: 10³–5×10⁷ M⁻¹s⁻¹
Dissociation Rate Constant (kd)	10⁻⁶–10⁻¹ s⁻¹
Affinity Range (KD)	fM–mM
Minimum Detectable Mass	100 Da
Short-Term Baseline Noise	<1 RU/min
Long-Term Baseline Noise	<2 RU/min
Cycle Time per Analysis	5–15 min
Data Display	Real-time kinetic binding curves, fitting results, calibration curves, concentration tables, kinetic data tables, epitope analysis plots, bar charts
Data Export Formats	Excel, TXT
Unattended Operation	Automated injection system supporting up to 48h continuous runs
Dimensions	740 × 450 × 485 mm
Weight	53 kg
Computer Interface	USB

The WeSPR One Auto combines automation, reproducibility, and ease of use in a compact SPR system. With its single-needle, dual-channel design and 48-hour unattended operation, it provides researchers with the tools to conduct efficient, reliable, and cost-effective molecular interaction studies. From small molecule screening to therapeutic antibody development, the system delivers consistent results while reducing hands-on time.

WeSPR One Auto Biomolecular Interaction Analyzer

Application Areas

Features

Specifications

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