Vibrio Fluvialis Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0142-ZJF)

Highlights

Plug-and-play PCR kit—no extra preparation needed.
High target fidelity thanks to conserved sequence alignment.
Maximum sensitivity with minimized background noise.

Cat Number: STEM-SLSR-0142-ZJF

Application: For qualitative and quantitative analysis of Vibrio Fluvialis

Model: 25107




Description

This kit offers a sensitive and specific method for detecting Vibrio Fluvialis using SYBR Green-based real-time PCR. Designed with primers targeting conserved regions, the assay produces fluorescence signals correlating with amplification levels. The real-time monitoring capability ensures accurate qualitative and quantitative results.

Specification

Kit Components:
Vibrio Fluvialis Amplification Mix: 50 µL × 1 tube
Vibrio Fluvialis Reaction Mix: 950 µL × 1 tube
Vibrio Fluvialis Positive Control: 50 µL × 1 tube
Vibrio Fluvialis Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Features

Plug-and-play PCR kit—no extra preparation needed.
High target fidelity thanks to conserved sequence alignment.
Maximum sensitivity with minimized background noise.
Comes with benchmark positive control for result assurance.
One-step fluorescence PCR avoids contamination.
For non-clinical research use only.

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