Variation Analysis of Biopharmaceuticals by Peptide Mapping (CAT#: STEM-B-0012-ZJF)

Introduction

Peptide mapping is an analytical technique widely used in the biopharmaceutical industry throughout the development of therapeutic proteins, primarily for the identification and verification of protein primary structure (amino acid sequence and chemical modifications). For recombinant protein drugs, peptide mapping analysis can be used to identify and characterize primary structures. In addition, the drug discovery process, various post-translational modifications (PTMs) produced by antibodies, degradation of protein molecules before storage, and contamination of protein samples, etc., directly affect the efficacy, stability and safety of drugs. Peptide map analysis is also the manufacture of these Process monitoring and Quality Assurance/Quality Control (QA/QC) are important techniques to ensure that there are no undesired changes in the product. Peptide mapping identifies proteins by first determining the molecular weights of the peptides and then matching those peptide masses to the protein's calculated theoretical mass.<br />This service provides a liquid chromatography and tandem mass spectrometry (LC-MS/MS)-based peptide mapping method for variation analysis of biopharmaceuticals (Peptide map analysis was used to determine disulfide bond positions, unpaired cysteines, free thiol groups, and to detect the occurrence of disulfide bond shuffling.).