TSKgel Phenyl-3PW(20) HIC Medium, 20 µm (CAT#: STEM-BC-3237-ZJF)

Highlights

High oligonucleotide capacity

Aqueous purification

DMT-on/DMT-off separation

Cat Number: STEM-BC-3237-ZJF

Application: for aqueous purification of oligonucleotides and peptides

Model: TSKgel Phenyl-3PW(20)




Description

This hydrophobic interaction medium is designed for purification of oligonucleotides, peptides, and intermediate-size proteins. It uses phenyl-functionalized particles with a 20 µm particle size. The medium provides high adsorption capacity for oligonucleotide processing. Its capacity is stated to be approximately 2.5 times that of an earlier hydrophobic interaction medium. DMT-on and DMT-off oligonucleotides can be separated in an aqueous system. Organic solvents are not required for the stated purification process. DMT deprotection may be completed directly inside the chromatography column. The medium supports lower-cost and efficient downstream oligonucleotide purification.

Specification

Chromatography mode: Hydrophobic interaction
Ligand: Phenyl
Particle size: 20 µm
Package size: 25 mL
Catalog number: 23566
Relative capacity: Approximately 2.5 times the earlier HIC medium
Mobile-phase mode: Aqueous, without required organic solvent
Applications: Oligonucleotides, peptides, and intermediate-size proteins
Additional function: In-column DMT deprotection

Features

High oligonucleotide capacity
Aqueous purification
DMT-on/DMT-off separation
In-column deprotection
Phenyl HIC selectivity
Reduced organic-solvent use
20 µm particles
Suitable for peptide purification

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